| Primary Identifier | MGI:3830850 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Twist2 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A targeting vector was designed to replace the first exon of the targeted gene with a cre cDNA sequence and an frt-flanked PGK-neo cassette. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Chimeric mice were bred to generate mutant mice. Mutant mice were then bred to FLPe mice (C57 genetic background) to remove the selection cassette. |
