| Primary Identifier | MGI:3841125 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Clec9a |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6J)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | The expression of NK1.1 (Klrb1c) is linked to Clec9a deficiency in homozygous mice, indicating that the homologous recombination step targeted the chromosome of C57BL/6J origin. |
| molecularNote | A farnesylated form of EGFP was inserted in frame with the two first amino acids of the gene locus. Half of the exon 1 and the entire exon 2 were eliminated with transcription following EGFP terminated by a strong polyadenylation signal. A loxP flanked neomycin cassette was included downstream of EGFP for targeting purposes. Gene inactivation was confirmed by a lack of protein expression by homozygote splenic CD8alpha+ dendritic cells. GFP expression was detected in the same population of dendritic cells. |
