| Primary Identifier | MGI:3844570 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Agr2 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 2 and 3 were floxed by insertion of a loxP site upstream of exon 2 and the addition of a FRT-neo-loxP-FRT-loxP cassette downstream of exon 3. The neo cassette was subsequently removed from correctly targeted ES cells by transient expression of FLPe recombinase. Mice carrying the floxed allele were crossed with transgenic mice that express cre recombinase in the germline to create a null allele. Gene inactivation was confirmed by RT-PCR analysis on intestinal extracts. |
