| Primary Identifier | MGI:3846097 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rffl |
| Inheritance Mode | Recessive | Transmission | Germline |
| Strain of Origin | 129S2/SvPas | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The first coding exon of the gene is interrupted by a Betageo (beta-galactosidase/neomycin resistance fusion gene) reporter/selection cassette, resulting in the in-frame joining of the Betageo coding sequence to the mouse genomic sequence encoding the first 14 amino acids of the endogenous protein. Whereas wild-type mouse embryonic fibroblasts (MEFs) express RFFL mRNA at levels easily detected by RT-PCR analysis, MEFs from embryos homozygous for this mutation produce no detectable transcript. |
