Primary Identifier | MGI:4422170 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Stk3 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 5 and an frt flanked neo cassette and second loxP site were inserted downstream of exon 6. Cre mediated recombination then removed exons 5 and 6 (encoding the kinase domain) and the neo cassette. Western blot analysis confirmed the absence of protein expression in homozygous embryos. |