Primary Identifier | MGI:4430285 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Epha4 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Two loxP sites were inserted flanking exon 3. Preceding the floxed exon 3, a reporter/selection cassette (SA-mCFP-Neo) flanked by two Frt sites, containing a splice acceptor, a membrane-targeted cyan fluorescent protein reporter gene and a neo cassette, was inserted. The targeted allele was bred to an FLP deleter mouse to excise the SA-mCFP-Neo cassette, resulting in a floxed exon 3. |