| Primary Identifier | MGI:4431363 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Cort |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | true | Is Wild Type | false |
| Project Collection | Neuroscience Blueprint cre |
| molecularNote | A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), a cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cortistatin locus (Cort). This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to generate the colony and remove the neo selection cassette. The FLPe transgene has been bred out of the line. |
