Primary Identifier | MGI:4441413 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Myo1e |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP-flanked neomycin resistance cassette was introduced to intron 4 and an additional loxP site was placed in intron 3. Cre-mediated recombination resulted in germline deletion of exon 4 and the neomycin cassette. The absence of protein expression was confirmed by western blot analysis on kidney and spleen extracts. |