| Primary Identifier | MGI:4451926 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Phlpp2 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line HEPD0619_1_C08 was successfully used to make chimeric mice. Germline transmission was accomplished. Cell lines HEPD0619_1_F08, HEPD0619_1_G08 and HEPD0619_1_H05 either failed the homologous recombination test or did not have germline transmission. J:231241 |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 110630450 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 110631337. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
