| Primary Identifier | MGI:4820294 | Allele Type | Targeted |
| Attribute String | Conditional ready, Modified isoform(s), Null/knockout, Reporter | Gene | Hnrnpr |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Alternative splicing of exon 2 of the Hnrnpr pre-mRNA generates two mRNA isoforms with a start codon in either exon 2 or exon 4, giving rise to two protein isoforms that differ at the Nâterminus. Special note about the mutation generated in mutant cell line EPD0609_5_A12: Analysis of protein expression in brain lysates of P5 mice, using an antibody against the C-terminal domain, revealed that the full-isoform of Hnrnpr (hn-RNP R-FL) is absent, while the truncated isoform lacking the N-terminal acidic domain (hnRNP R-deltaN) is still expressed and even increased in homozygous mutant brains by a compensatory mechanism. Absence of hnRNP R-FL isoform and expression of hnRNP R-deltaN is also detectable in the heart and kidney of homozygous mutant mice, without any upregulation of hnRNP R-deltaN in these tissues (J:317395). |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 136043191 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 136043993. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
