Primary Identifier | MGI:4840469 | Allele Type | Targeted |
Gene | Rad21 | Transmission | Germline |
Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Three consecutive TEV protease recognition sites with a novel BamHI restriction site were inserted between P475 and P476 of exon 11. At the stop codon an additional BamHI was inserted as well as a C-terminal c-Myc epitope tag with a stop codon. A floxed puro cassette was inserted downstream of exon 12. Cre mediated recombination removed the puro cassette. |