| Primary Identifier | MGI:4841604 | Allele Type | Targeted |
| Attribute String | Conditional ready, Hypomorph, Null/knockout, Reporter | Gene | Hacd2 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line HEPD0663_6_E11 was not successfully used to make chimeric mice. Germline transmission was not accomplished. J:333944 Cell lines HEPD0663_6_A10 and HEPD0663_6_G09 were successfully used to make chimeric mice. Germline transmission was accomplished. J:333944 Special note about mutant cell lines HEPD0663_6_A10 and HEPD0663_6_G09: The mutation generated in HEPD0663_6_A10 or HEPD0663_6_G09 results in a hypomorphic allele. RT-qPCR analysis confirmed reduced levels of mRNA in homozygous mutant pups, with the strongest reduction observed in liver (- 88%), kidney (- 84%), BAT (- 85%) and heart (- 83%), and a less pronounced reduction observed in skeletal muscle (- 54%). |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 34845737 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 34846568. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
