| Primary Identifier | MGI:5000016 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gpr55 |
| Transmission | Germline | Strain of Origin | 129S5/SvEvBrd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was constructed to replace part of exon 2 of the Gpr55 gene (containing the entire coding region) with a selection cassette. Homologous recombination was carried out in 129SvEvBrd-derived ES cells, followed by injection of targeted ES cells into blastocyts. Loss of Gpr55 mRNA was confirmed by RT-PCR which demonsrated lack of Gpr55 cDNAs in homozygous animals. |
