Primary Identifier | MGI:5007937 | Allele Type | Targeted |
Attribute String | Recombinase | Gene | Mcpt8 |
Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A 4.8 kb reporter cassette containing YFP, IRES site, humanized cre recombinase, bGH polyA, and a loxP-flanked neo selection marker was inserted at the Mcpt8 start site via homologous recombination in ES cells contain PrmCre (PC3 cells with Tg(Prm-cre)70Og). Upon crossing male chimeras to wild-type females, the loxP-flanked neo was deleted from the male germline by cre recombination, producing the final knock-in allele. |