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Allele : Gt(ROSA)26Sor<tm7.1(CAG-EGFP/RNAi:Tyr)Maoh> gene trap ROSA 26, Philippe Soriano; targeted mutation 7.1, Masato Ohtsuka
Primary Identifier  MGI:5056538 Allele Type  Targeted
Attribute String  Conditional ready, Knockdown, Reporter, RMCE-ready Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  129P2/OlaHsd
Is Recombinase  false Is Wild Type  false
molecularNote  Recombinase-mediated cassette exchange (RMCE) replaced the expression cassette between the JT15 and lox2272 sites of Gt(ROSA)26Sortm1Maoh with (5' to 3') an IRES, lacZ, polyadenylation sequence, CAG promoter, hygro gene, polyadenylation sequence, FRT site, polyadenylation sequence, and CAG driven fusion of EGFP and a short hairpin RNA targeted to Tyrosinase. The RNAi target sequences within the Tyr gene are "ctggaaggatttgccagtccac"and "gaactgccaatttcagcttta". Flp-mediated recombination removes all but the CAG promoter RNAi construct flanked by an FRT site and the lox2272 site.
  • mutations:
  • Insertion
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1 Feature

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1 Mutation Involves

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