| Primary Identifier | MGI:5300919 | Allele Type | Targeted |
| Attribute String | Transactivator | Gene | Pparg |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = ZX1, a mutant A17 line carrying an inducible cassette exchange allele at Hprt. The A17 ES cell line used to make the ZX1 cells is a mutant E14TG2A parental ES cell line carrying an inserted rtTA sequence at the Rosa26 locus. |
| molecularNote | A targeting construct was electroporated into ES cells and by homologous recombination, a tTA-Frt-neo-Frt (tTA = tetracycline transactivator) sequence was inserted into the second coding exon of Pparg, creating an in-frame fusion between the amino terminus of Pparg and tTA. Correctly targeted ES cells were used to generate mice. The neo resistance cassette was removed from the germline by crossing Pparg mutants with FLPe-expressing mice. |
