| Primary Identifier | MGI:5302027 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mir21a |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted 3' of exon 12 of the Vmp1 gene and 5' of the Mir21 sequence. Mir21 was then followed by an FRT flanked PGK-puro/deltaTK selection cassette and a second loxP site. Mir21 and the selection cassette were then excised by crossing properly targeted mice to Tg(EIIa-cre)C5379Lmgd mice. Northern blot and PCR analysis confirmed the absence of MIR21. |
