Primary Identifier | MGI:5302222 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Lzts2 |
Transmission | Germline | Strain of Origin | (C57BL/6NTac x 129S6/SvEvTac)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP and FRT flanked neo cassette was inserted upstream of exon 2 and a loxP site was inserted downstream of exon 3 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 2 and 3. Western blot analysis confirmed the absence of protein expression in homozygous embryos. |