| Primary Identifier | MGI:5302414 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Pclaf |
| Transmission | Germline | Strain of Origin | B6(Cg)-Tyr<c-2J> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of the translation initiation site in exon 1. An additional loxP site was inserted downstream of exon 2. Cre-mediated recombination removed the neo cassette, 3' portion of exon 1 downstream of the translation initiation codon, intron 1, and exon 2. Western blot analysis confirmed the absence of protein expression in thymocytes. |
