| Primary Identifier | MGI:5538716 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Slirp |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was replaced with loxP-flanked cDNA for exons 2 to 4, with splice acceptor site at the 5' end and a polyA signal at the 3' end. Cre recombinase removed the loxP-flanked sequence, leaving behind a loxP site and downstream artificial exon with a splice acceptor, Flag epitope coding sequence, stop codon and c-fos sequence. Western blot analysis confirmed the absence of wild-type protein in testis lysates from homozygous mice. |
