| Primary Identifier | MGI:5546597 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Prmt5 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Mice were generated from cell line EPD0160_2_A08 in J:201152 |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 54750459 of Chromosome 14 upstream of exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 54751393. The critical exon 7 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Flp mediated recombination removed the lacZ cassette creating a floxed allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |
