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Allele : Pkm<tm1.1Mgvh> pyruvate kinase, muscle; targeted mutation 1.1, Matthew G Vander Heiden
Primary Identifier  MGI:5547750 Allele Type  Targeted
Attribute String  Conditional ready, Modified isoform(s), No functional change Gene  Pkm
Transmission  Germline Strain of Origin  129S6/SvEvTac
Is Recombinase  false Is Wild Type  false
molecularNote  A targeting vector was designed to contain an FRT-flanked neomycin cassette upstream of exon 9, and loxP sites that flank exon 10 which is alternatively spliced only in the Pkm2 isoform. Mutant mice were bred with mice expressing FlpO recombinase (Gt(ROSA)26Sor) to remove the neomycin selection cassette. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 10 deleted in the cre-expressing tissues: pyruvate kinase M2 isoform expression is eliminated, while pyruvate kinase M1 isoform expression is unchanged.
  • mutations:
  • Insertion
  • synonyms:
  • Pkm2<fl>,
  • Pkm2<fl>
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

2 Carried By

0 Driven By

52 Publication categories





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