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Allele : Pld1<tm1.1Gdp> phospholipase D1; targeted mutation 1.1, Gilbert Di Paolo

Primary Identifier  MGI:5556064 Allele Type  Targeted
Attribute String  Null/knockout Gene  Pld1
Transmission  Germline Strain of Origin  C57BL/6 x 129/SvJ
Is Recombinase  false Is Wild Type  false
molecularNote  A loxP site was inserted upstream of exon 13. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 14. This allele is created by using either Cre-mediated recombination to remove exon 13, exon 14 and the selection cassette or Flp-mediated recombination to remove the FRT-flanked neo cassette, followed by Cre-mediated recombination to remove exons 13 and 14 . Western blot analysis confirmed the absence of protein expression in the liver and mouse embryonic fibroblasts.
  • mutations:
  • Intragenic deletion,
  • Insertion
  • synonyms:
  • Pld1<tm2.1Mafr>,
  • Pld1<tm2.1Mafr>
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

0 Driven By

21 Publication categories