Primary Identifier | MGI:5565201 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Stag3 |
Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
Project Collection | KOMP-CSD |
molecularNote | The L1L2_Bact_P cassette was inserted at position 138286583 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette created a reporter knockout mouse, and subsequent Cre-meidated excision deleted the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |