| Primary Identifier | MGI:5581675 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rcor1 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was introduced to intron 3 and an FRT-PGK-Neo-pA-splice donor-FRT-loxP cassette was placed in intron 4. Flpe-mediated recombination removed the FRT-flanked neo cassette leaving loxP sites on either side of exon 4. Cre-mediated recombination removed exon 4 and introduced a premature stop codon. Western blot analysis demonstrated that no detectable protein was present in homozygous E13.5 embryos. |
