| Primary Identifier | MGI:5585631 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tyr |
| Inheritance Mode | Recessive | Transmission | Germline |
| Strain of Origin | C57BL/6NTac | Is Recombinase | false |
| Is Wild Type | false |
| description | The frt-flanked selection cassette was deleted in vitro in the targeted ES cells by transient transfection with a flpe expression vector. (J:213411) |
| molecularNote | Guanosine was replaced by cytosine at nucleic acid position 308 of the tyrosinase gene, in the first exon, replicating the inactivating Tyrc mutation that results in substitution of serine for cysteine at amino acid position 103 of the tyrosinase protein; a puromycin resistance cassette flanked by frt sequences was inserted into intron 1. The selection cassette was excised by flp recombinase, leaving a single frt site in the intron. |
