| Primary Identifier | MGI:5588124 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence, Transactivator | Gene | S1pr1 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed with two fusion proteins separated by an internal ribosome entry site (IRES). One fusion protein contains a tobacco etch virus (TEV) protease recognition sequence (tevs) fused to a tetracycline-regulated transactivator (tTA or "Tet-Off") sequence. The second contains a murine arrestin, beta 2 (Arrb2) gene fused to the TEV protease. This construct was inserted at the 3 end of exon 2 of the sphingosine-1-phosphate receptor 1 (S1pr1) gene and was followed by a polyadenylation sequence and a loxP-flanked neomycin (neo) resistance cassette. Cre-mediated recombination removed the selection cassette. |
