| Primary Identifier | MGI:5605760 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Rnf216 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | Cre-mediated excision of the parental Rnf216tm1a(EUCOMM)Wtsi allele resulted in the removal of the promoter-driven neomycin selection cassette and critical exons 4 and 5 leaving behind the inserted lacZ reporter sequence. Western blot analysis confirmed the absence of protein expression in the brain and testes of homozygous mutant mice. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. |
