Primary Identifier | MGI:5620763 | Allele Type | Targeted |
Attribute String | Inducible, Null/knockout, Recombinase | Gene | Lhx9 |
Transmission | Germline | Strain of Origin | C57BL/6J x 129S6/SvEvTac |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | The targeting construct replaced exon 4 with an EGFP, inducible cre recombinase fusion gene (EGFP/cre/ERT2) and an FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. The EGFP could not be detected by direct fluorescence or anti-GFP antibody staining. Expression of the EGFP/cre/ERT2 fusion protein is detected by anti-estrogen receptor antibody staining in adult retinal neurons. |