| Primary Identifier | MGI:5647876 | Allele Type | Targeted |
| Attribute String | Modified isoform(s) | Gene | F3 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The open reading frame was replaced with one encoding the soluble alternatively spliced form and a loxP flanked neomycin resistance cassette. After homologous recombination into ES cells, the floxed neomycin resistance cassette was removed by transfection with a Cre expression plasmid. mRNA expression of the soluble form was 5.9-fold higher in homozygous mutant embryos relative to wild-type embryos, whereas mRNA expression of the full-length form was undetectable in homozygous mutant embryos. Western blot analysis revealed comparable protein expression of the soluble form in E9.5 homozygous mutant and wild-type embryos. |
