| Primary Identifier | MGI:5660837 | Allele Type | Targeted |
| Attribute String | Inducible, Null/knockout, Recombinase, RMCE-ready | Gene | Srd5a2 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| description | The exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-derived JM8A3.N1.C2 knockout first reporter embryonic stem (ES) cells obtained from the Knockout Mouse Project (KOMP) consortium containing clone HEPD0799_3_G07. Recombined ES cells lack the neomycin cassette removed and are resistant to puromycin. No EGFP fluorescence is seen in these mice. |
| molecularNote | An exchange vector was designed to be inserted downstream of exon 1 of the gene using dual-recombinase mediated cassette exchange (dRMCE). Specifically, the exchange vector contained (from 5 to 3) an FRT site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) sequence, a viral T2A oligopeptide, and a Cre-ERT2 sequence (Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain). A rox-flanked puromycin resistance cassette and a loxP site were inserted at the 3' end of the exchange vector. |
