| Primary Identifier | MGI:5707512 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence, Reporter | Gene | Dicer1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129P2/OlaHsd)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele encodes the wild-type dicer protein with N-terminal Flag-HA-HA epitope tags; the DNA sequence encoding the tags was inserted immediatedly downstream of the translation initiating ATG. A single loxP site in intron 2 marks the site of Cre recombinase excision of a loxP-flanked neomycin resistance cassette. Immunofluorescence analysis of testis sections using anti-HA antibodies demonstrated high expression of the tagged protein in mitotic spermatogonia and the early meiotic stages, increasing to a maximum in early pachytene spermatocytes and subsequently diminishing but remaining detectable in later stages of spermiogenesis. |
