| Primary Identifier | MGI:5762595 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Csnk1a1 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Cell line EPD0125_5_H05 was successfully used to make chimeric mice. Germline transmission was accomplished. J:216977 |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 61700893 of Chromosome 18 upstream of the critical exon3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 61701699. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele which removes the neomycin/lacZ cassette. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. |
