| Primary Identifier | MGI:5763138 | Allele Type | Endonuclease-mediated |
| Gene | Psmb11 | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| description | Guide RNA and hCas9 mRNA were injected into fertilized eggs from either (C57BL/6 x DBA/2)F1 x C57BL/6-Psmb11Tn or C57BL/6 x C57BL/6-Psmb11Tn. |
| molecularNote | CRISPR technology was used to generate a G to A nucleotide substitution that results in the amino acid substitution of arginine for glycine at position 220 (G220R). Founder 2 is heterozygous for the expected point mutation without any additional mutations. Founder 3 is heterozygous for the expected point mutation and an additional single base pair insertion. Founder 21 is heterozygous for the expected point mutation and an additional 10 bp deletion. Founder 22 is heterozygous for the expected point mutation and an additional 8 bp deletion. Founder 28 is heterozygous for the expected point mutation without any additional mutations. The pound symbol (#) is used for the pool of founders 3, 21 and 22. |
