| Primary Identifier | MGI:5781010 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Enho |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 2. An additional loxP site was inserted in exon 2 downstream of the coding sequence. Cre-mediated recombination removed the loxP-flanked region, including the coding sequence and the selection cassette. RT-PCR analysis revealed absence of RNA expression in the liver, whole brain, and skeletal muscle of homozygous mutant mice. Absence of serum levels in chow-fed conditions was confirmed by ELISA. |
