Primary Identifier | MGI:5792890 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Ddx60 |
Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
Project Collection | EUCOMM |
molecularNote | The L1L2_Bact_P cassette was inserted at position 62407097 of Chromosome 8 upstream of the exon 9 (Build GRCm39; exon 10 in J:228452). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 9 (or exon 10 J:228452) at position 62407909. Cre expression resulted in the deletion of the targeted exons and neomycin selection cassette, creating a reporter knockout mouse. |