| Primary Identifier | MGI:5897622 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Igsf11 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| description | Cell line EPD0297_2_A07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:234439 |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 38828713 of Chromosome 16 upstream of the critical exons 3 through 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 3 through 5 at position 38829597. The critical exons 3 through 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis confirmed the absence of protein expression in the brain. |
