| Primary Identifier | MGI:5908153 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Gnrhr |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP-flanked exon 3 with a polyA sequence, FRT-flanked neomycin selection cassette, and a modified exon 3 encoding the C-terminus of mouse Gnrhr fused in-frame with the intracellular cytosolic tail (Ctail) of the chicken GNRHR replaced the endogenous exon 3. The neomycin selection cassette was removed via flp-mediated recombination. Cre-mediated recombination removed the floxed exon 3 leaving an exon 3 composed of the mouse C-terminus fused with the chicken intracellular cytosolic trail. |
