Primary Identifier | MGI:5909077 | Allele Type | Targeted |
Attribute String | Null/knockout, Recombinase, Reporter | Gene | Bhlha9 |
Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A vector was constructed for replacement of the endogenous Bhlha9 locus with the Venus-Cre and FRT flanked PGK-Neo cassette via homologous recombination in embryonic stem cells. Upon recombination, although genome mutation was confirmed by Southern blotting and PCR, Venus-Cre protein expression in limb bud was not detected. |