| Primary Identifier | MGI:5912122 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Shtn1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences TTACACCTCCCCATTAGAGC, GCCTGGGCTTCTGTGCAACT, AGCTTTTTAGTCATAGCCAA and ATTATCTGAGATTTAATCAA, which resulted in a 449 bp deletion beginning at Chromosome 19 negative strand position 59,042,109 bp and ending after 59,041,661 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000288532 (exon 4) and 354 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 57 and early truncation 2 amino acids later. |
