| Primary Identifier | MGI:6113637 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Penk |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac or C57BL/6J |
| Is Recombinase | true | Is Wild Type | false |
| description | "The targeting vector was electroporated into F1 hybrid of 129S6 x C57BL/6J ES cells derived by Janelia Transgenic Facility." (J:253245) |
| molecularNote | The targeting vector was constructed using recombineering techniques. An 8,528 bp genomic DNA fragment containing the last exon of the mouse Penk gene was retrieved from BAC clone RP23-RP23- 365K8 to a vector containing the DT gene. A cassette of IRES-Cre frt-PGKNeo-frt was inserted between stop codon TGA and 3' UTR. The neo cassette was removed by breeding germline chimeras with FLP1 expressing homozygous females. |
