| Primary Identifier | MGI:6150284 | Allele Type | Targeted |
| Attribute String | Conditional ready, Modified isoform(s), Null/knockout | Gene | Spp1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 1 was replaced with an FRT-flanked STOP cassette and a modified exon 2 lacking the 45 nucleotides correspondihng to the signal sequence. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of exon 3. Cre-mediated recombination is required to achieve expression of the intracellular isoform of the protein. This allele cannot produce the soluble isoform. |
