| Primary Identifier | MGI:6149981 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Zswim6 |
| Transmission | Germline | Strain of Origin | (C57BL/6NTac x 129S6/SvEvTac)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A FRT-LoxP-Neomycin-FRT-LoxP selection cassette was inserted downstream of exon 4 and the third single LoxP site was inserted upstream of exon 4. The region flanked by the second and third LoxP sites is approximately 569 bp. Exon 4 encodes amino acids 337-386 and deletion of this sequence results in the creation of a premature stop codon. The neomycin selection cassette was removed via FLP-mediated recombination, leaving loxP sites flanking exon 4. Exon 4 was susequently removed via Cre-mediated recombination. In situ hybridization and RT-PCR analysis confirmed that this allele lacks expression of exon 4. qPCR analysis revealed that the medial ganglionic eminence of homozygous mutants expresses transcript downstream of the deleted region at ~35% of control levels at E13.5. |
