| Primary Identifier | MGI:6150833 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Pgp |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site downstream of exon 2. Also inserted downstream of exon 2 was a cDNA for the endogenous gene where a point mutation was engineered to change aspartate codon 34 into an asparagine codon (p.D34N or p.Asp34Asn). The neo cassette was removed through subsequent flp-mediated recombination and exons 1-2 (comprising the entire gene) were deleted through subsequent cre-mediated recombination. This leaves the mutated cDNA which codes for a kinase-dead version of the protein. |
