Primary Identifier | MGI:6156086 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Lhx3 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac or C57BL/6J |
Is Recombinase | false | Is Wild Type | false |
description | "Linearized Lhx3ckoGFP targeting construct was electroporated into C57BL/6J-129S6 hybrid embryonic stem cells (ESCs)." (J:261409) |
molecularNote | A FRT site, GFP, loxP site, neomycin resistance cassette, FRT and a loxP site were inserted upstream of exon 3 and a third loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the neomycin and GFP. Cre-mediated recombination removed exons 3-5. |