| Primary Identifier | MGI:6202047 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence | Gene | U2af1 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = HG3 - (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1 |
| molecularNote | The targeting vector is designed with a loxP site downstream of the endogenous exon 1 of the gene followed by exons 2-8. A transcriptional STOP sequence (3 repeats of SV40 late poly A sequence) followed by an FRT-flanked neomycin selection cassette and a second loxP site were inserted downstream of the exon 2-8 sequence of the targeting construct. A missense mutation (TCT > TTT) was created in the endogenous exon 2, resulting in a serine to phenylalanine mutation at position 34 (S34F). |
