Primary Identifier | MGI:6256965 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Daxx |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A FRT site flanked targeting vector, with a loxP site at the downstream end and containing a PGK-neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon I (also called exon 2, when the first non-coding exon is included in the numbering scheme) of the targeted gene, and another loxP site was inserted upstream of exon III (also called exon 4, when the first non-coding exon is included in the numbering scheme). The PGK-neo selection cassette was removed by Flp-recombinase mediated excision. Exon II is flanked by loxP sites. Cre-mediated recombination removed exon II. |