| Primary Identifier | MGI:6283680 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Zfp296 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:255491 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
| molecularNote | A targeting vector was designed to insert an IRES-beta-geo-pA cassette into exon 3 which encodes all of the zinc-finger domains. Western blot analysis confirmed the absence of protein expression in the adult testis of homozygous mutant mice. |
