Primary Identifier | MGI:6384215 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Pfn3 |
Strain of Origin | (C57BL/6 x DBA)F2 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 genome editing was used to delete 254 bp causing a frameshift in the reading frame that leads to a premature stop codon after amino acid 6. qRT-PCR, Western blotting, and immunohistochemistry confirmed the deletion in the testes of homozygous mutant mice. |