| Primary Identifier | MGI:6358416 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nsmf |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted into the 5UTR of exon 1, an FRT-flanked PGK-neomycin selection cassette was inserted after exon 2, followed by a loxP site and a third loxP site downstream of exon 3. Flp-mediated recombination removed the selection cassette and cre-mediated recombination deleted exons 1-3. Western blot analysis confirmed absence of protein. |
